In this short video, Mike Gibbs explains the CRISPR-Cas9 gene editing method, including a brief description of the different types of Cas enzymes.
CRISPR-Cas9 promises to be the most efficient method of gene editing available today. It requires only one enzyme and an easily-edited guide RNA sequence, or gRNA. The gRNA consists of a Cas9-specific section that binds to the Cas enzyme, a structural section that forms a pinhole loop, and a targeting section of RNA specific to the gene that the designer wants to edit. The targeting section of the RNA hybridizes with the DNA strand that requires editing, and once positioned, the Cas9 enzyme goes to work.
Despite copyright issues, the CRISPR-Cas9 method is simple to use and versatile in its function. It promises to make gene editing more accessible and cheaper, allowing everyone from major companies and amateur biohackers the opportunity to test their creativity and partake in the genetic revolution.
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